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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important purpose in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been explained. Two more splice variants have been claimed but specific nucleotide sequence details isn't still accessible.
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Blocking peptides are peptides that bind especially towards the focus on antibody and block antibody binding. These peptide generally consists of the epitope recognized from the antibody.
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This guide outlines additional doable optimization of mobile and immunoassay parameters to be certain the very best success are...
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This information outlines more probable optimization of cellular and immunoassay parameters to be certain the best possible final results are...
Antibodies sure to the blocking peptide no more bind into the epitope on the concentrate on protein. By comparing the staining from the blocked antibody versus the antibody alone, you can see which staining is specific.
Concentrate on Info Cyclin E belongs to your highly conserved cyclin relatives, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic event. Cyclin E forms a posh with and features for a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S phase boundary and it is degraded as cells development through S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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